mtSEEK®  Mitochondrial Genome Sequencing with Heteroplasmy Analysis

Courtagen’s mtSEEK® test uses Next Generation Sequencing to sequence the 16,569 nucleotide bases that make up the 37 genes of the mitochondrial genome.

The analysis and interpretation of a patient’s nuclear mitochondrial exome is valuable for obtaining a definitive diagnosis, estimating prognosis, guiding treatment choices, and determining recurrence risks.

Assay Design and Performance Features

Courtagen’s proprietary DREAM PCR technique provides robust contamination control – ensuring that contamination in the laboratory is not mistaken for heteroplasmy.

Two Long Range PCR libraries cover the entire mitochondrial genome – maximizing sequencing accuracy and variant detection sensitivity.

Deep sequencing coverage (>2,000x) and use of 2x250bp sequencing reads delivers highly accurate sequencing and mapping results – reducing nuclear mtDNA (numt) noise and providing sensitivity of heteroplasmy detection as low as 5%.

Product Information Sheet: 

mtSEEK® Mitochondrial Genome Sequencing with Heteroplasmy Analysis

Courtagen's Unmatched Customer Support

Turn Around Time: 3-4 weeks. Results are delivered in weeks, not months.

Saliva Sample: DNA for sequencing is reliably extracted from a single saliva sample. No blood draw or muscle biopsy required. (Whole blood and tissue are accepted, as requested.)

Insurance Assistance: Courtagen works with patients, physicians, and insurance carriers to pre-approve each test. Courtagen will bill the insurance company, and is willing to handle an appeal process as needed.

Courtagen Care Financial Assistance Program: Provides financial assistance to patients based on demonstrated financial need. A qualified applicant may be asked to contribute between $0 and a maximum cost sharing of $200 depending on determination of financial need.

Online Portal: A secure physician online portal is available for ordering genetic tests and accessing patient reports when completed.

Genetic Counselors: Available to address physician’s questions regarding Courtagen test results. Contact us at

Clinical Experience: Courtagen’s Medical Director, Laboratory Director, and variant science team have over 25 years of experience in the treatment and genetic interpretation of neurological and metabolic disorders.

Reports: Utilizing Courtagen’s customized Ziphyr® informatics pipeline and thorough clinical evaluation, each report is provided in a concise format with interpretation and recommendations for consideration.

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